Anaerobic respiration in yeast Essay

AIM See the effect of temperature in anaerobiotic respiration of barm by numbering carbon dioxide bubbles.HYPHOTESIS Anaerobic respiration in yeast will decrease as temperature increases.VARIABLES Independent TemperatureDependent Rate of anaerobic respiration in yeastFix Volume of sugar solution (40ml) , Concentration of sugar solution, yeast mass (2g), volume of solution of yeast & sugar all together (20ml)MATERIALSDelivering underground2 test tubes2 beakersBunsen BurnerTap WaterThermometer (0.1)Electronic BalanceSpatulaSugar SolutionYeastWaterMeasuring Cylinder part1. Mea incontestable 40 ml of sugar solution with the measuring tube. Place the 40 ml on the big beaker2. Measure 2 g of yeast using the electric balance. Place the 2 g on the beaker with the sugar solution3. Stir the yeast-sugar solution (Solution A) using the glass stick until the yeast has all dissolved4. Separate Solution A into two 20 ml solutions (Solution A1 and Solution A2). To do so, measure 20 ml with the measuring tube and derriere them on a small beaker. Repeat the procedure with the other 20 ml.5. Prepare a water bath on the Styrofoam cup. Using the thermomether, make sure the water bath has a temperature of 45C.6. Light the Bunsen burner. Heat solution A1 to a temperature of 40C7. Using the measuring cylinder, measure 15 ml of Solution A1 and place them on one of the boiling tubes. Cover the boiling tube with the sales pitch tube and place the other end of the delivery tube into a test tube which contains tap water8. Count the number of CO2 bubblescoming egress of the delivery tube. Record the number of bubbles which came out each minute (checking time with the stopwatch) 9. Repeat from step 5 with Solution A2, but this time heat the solution up to a temperature of 100C instead of 40C.CONCLUSIONWe may realize that our aim was successfully achieved because wewere able to know the effect of anaerobic respiration in yeast in 2 temperatures 40 & a boiling temperature (90-100). If we see our results we may see that in test tube B, the one with the boiling water, hasn?t produced CO2 bubbles, there is no bubbles because yeast is boiled, so yeast dont breath because when it was boiled it was killed, that causes yeast to not to breath. By looking at the formula of anaerobic respiration in fungi (yeast) & bacteriaWe puke see that yeast contains glucose, because if the product is CO2 , the have to contain glucose. If we see graph 1 we can see that the line that indicates de results for water at 40 is increasing as time passes. As I already mentioned, at a boiling temperature yeast dont breath so we can say that as temperature increases less CO2 bubbles are produced, so less respiration is done by yeast.EVALUATION One way in which we may improve the experiment is by doing different ranges of temperatures, & changing the volume of yeast it would be interesting for other experiment. One of the main source of uncertainty in this experiment is the beholder because i f you confuse the number of bubbles the results may be different, in another way the observer could be the source of uncertainty is by when reading the thermometer placed into the water, you may confuse and the results will not be the same because yeast will be placed in another temperature.